1.1 This guide covers two distinct procedures, based on similar principles, for obtaining data concerning the adverse effects of a test material (added to dilution water) on oceanic bioluminescent dinoflagellates.
1.1.1 The endpoint for both procedures is based on a measurable reduction or inhibition in light output from the dinoflagellates. Both procedures are similar in that when bioluminescent dinoflagellates are exposed to toxicants, a measurable reduction in bioluminescence is observed from their cells following mechanical stimulation when compared to control cells. In the first procedure, cells of the bioluminescent dinoflagellate Gonyaulax polyedra can be tested over a range of up to seven days of exposure (or longer) to a toxicant. The second procedure uses another species, Pyrocystis lunula, for a 4 h test.
Product Details
- Published:
- 12/01/2012
- Number of Pages:
- 12
- File Size:
- 1 file , 150 KB
