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1.1 This practice covers procedures for obtaining data concerning toxicity and other effects of a test material to a multi-trophic level freshwater community.

1.2 These procedures also might be useful for studying the fate of test materials and transformation products, although modifications and additional analytical procedures might be necessary.

1.3 Modification of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparison of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting multi-trophic level tests.

1.4 This practice is arranged as follows:

Section
Referenced Documents2
Terminology3
Summary of Practice4
Significance and Use5
Apparatus6
Facilities6.1
Container6.2
Equipment6.3
Hazards7
Microcosm Components8
Medium8.1
Medium Preparation8.2
Sediment8.3
Microcosm Assembly8.4
Test Material9
General9.1
Stock Solution9.2
Nutrient Control9.3
Test Concentration(s)9.4
Test Organisms10
Algae10.1
Animals10.2
Specificity of Organisms10.3
Sources10.4
Algal Culture Maintenance10.5
Animal Culture Maintenance10.6
Section
Procedure11
Experimental Design11.1
Procedure
Inoculation11.2
Culling11.3
Addition of Test Material11.4
Measurements11.5
Reinoculations11.6
Analytical Methodology12
Data Processing13
Calculations of Variables from Measurements14
Statistical Analyses15
Acceptability of Test16
Interpretation of Results17
Report18
AnnexAnnex A1
Appendices
Relationship of MediaAppendix X1
Data SheetsAppendix X2
Statistical GuidanceAppendix X3

1.5 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.

1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 7.

Product Details

Published:
12/10/2002
Number of Pages:
37
File Size:
1 file , 620 KB
Redline File Size:
2 files , 1.2 MB